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DTNB (Sigma-Aldrich) was dissolved in 10× PBS (pH 7.4) to make a 4 mg ml −1 (10 mM) solution and subsequently diluted in assay buffer to a final concentration of 0.2 mM
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In total, we screened 63 genes in four metabolic states, corresponding to 2016 single and pair-wise genetic perturbations
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